Page 271 - 《华中农业大学学报（自然科学版）》2022年第5期

第 5 期 崔瑞霞 等：ACMO-co-DVB-co-NVP@MCM-41 的制备及其在豇豆农药固相微萃取中的应用 265

Preparation of ACMO-co-DVB-co-NVP@MCM-41 and its application
in solid-phase microextraction of pesticides in cowpea

CUI Ruixia,XIANG Xiaozhe,SUN Xin,ZHANG Xiaofan,LI Xiujuan
College of Food Science and Technology，Huazhong Agricultural University/

Key Laboratory of Environment Correlative Dietology，
Ministry of Education/Hubei Key Laboratory of Fruit & Vegetable Processing &
Quality Control，Wuhan 430070，China

Abstract ACMO-co-DVB-co-NVP@MCM-41 material was obtained by polymerizing divinylbenzene
（DVB），N-vinylpyrrolidone （NVP） and 4-acryloylmorpholine （ACMO） on MCM-41 mesoporous micro⁃
spheres to meet the requirements of detecting polar pesticides in cowpea. The material was characterized with

scanning electron microscope，Fourier transform infrared spectrometer，thermogravimetric analyzer and N2 ad⁃
sorption-desorption. Solid-phase microextraction（SPME）fibers were prepared from this material. A method for
determining methomyl，carbofuranandcyromazine in cowpea was established by direct immersion-SPME com⁃
bined with gas chromatography-nitrogen phosphorus detector. Under the optimized extraction conditions，the lin⁃
ear relationship of the three pesticides was good，the coefficients of determination were all greater than 0.99，

with the detection limits of 1.71-18.79 μg/kg and the quantification limits of 5.71-62.63 μg/kg. The recoveries of
3 pesticides ranged from 84.4% to 116.1%，and the relative standard deviations ranged from 2.2%-7.5%. It is
indicated that the method established has good sensitivity，accuracy and reliability，and can satisfy the maximum
residue limit（MRL）of pesticide in cowpea. Compared with the popular coating made from hydrophilic-lipophil⁃
ic balance particles，it has a large extraction capacity and a stronger extraction performance for strong polar sub⁃
stances，which will have a good application prospect in the extraction of non-targeted analytes.
Keywords hydrophilic-lipophilic balance particles；4-acryloylmorpholine；solid-phase microextraction；

pesticide residues；cowpea；detection method

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