Page 262 - 《华中农业大学学报（自然科学版）》2022年第5期

256 华 中 农 业 大 学 学 报 第 41 卷

中氟虫腈及其代谢物残留［J］. 食品科学，2021，42（14）：333- ［24］ 夏清华 .柑橘果实中有机磷类农药残留监测及其受加工处理的
338.GAO X，CHEN H，TAN M，et al.Determination of fipronil 影响研究［D］. 重庆：西南大学，2020.XIA Q H.The monitoring
and its metabolites in vegetables by gas chromatography-negative of organophosphorus pesticide residues in citrus and evaluation of
ion chemical ionization-mass spectrometry［J］. Food science， the impact of handling and processing［D］.Chongqing：Southwest
2021，42（14）：333-338（in Chinese with English abstract）. University，2020（in Chinese with English abstract）.

Determination of three pesticides residue in pakchoi with in vivo
solid-phase microextraction-gas chromatography

SUN Xin,ZHANG Dan,ZHANG Xiaofan,LI Xiujuan
College of Food Science and Technology，Huazhong Agricultural University/
Key Laboratory of Environment Correlative Dietology，Ministry of Education/
Hubei Province Key Laboratory of Fruit & Vegetable Processing & Quality Control，
Wuhan 430070，China

Abstract Three pesticides including chlorothalonil，chlorpyrifos and fipronil in pakchoi were sampled

and determined quantitatively by sampling rate calibrated in vivo solid-phase microextraction-gas chromatog⁃
raphy. The environmental behavior of pesticides including absorption，enrichment and dissipation in pakchoi
were studied. The results showed that the in vivo detection method was accurate，sensitive，fast and conve⁃
nient. Both light and temperature promoted the degradation of three pesticides in pakchoi. The application
method affected the absorption of pesticides，and the absorption rate of the three pesticides by foliar spray⁃
ing of pakchoi was faster. The culture method affected the degradation of pesticides，and the rate of pesti⁃
cide degradation in the soil-cultured pakchoi was faster than that of the pakchoi cultured in water. Posthar⁃
vest storage had an effect on the degradation of pesticides. Storage temperature had little effect on the dissi⁃
pation rate of chlorothalonil，while low temperature inhibited the dissipation of chlorpyrifos and fipronil.
The results showed that among the three pesticides，chlorothalonil had the worst stability，fipronil had the
best stability at 4 ℃，and chlorpyrifos was relatively stable at room temperature and 4 ℃. It will provide an
analytical method for tracking pesticides in living plants and an insight into the environmental behavior in
complex biological systems. It will be helpful to better understand the characteristics of pesticide residues in
pakchoi and to reduce the potential health risks in human.
Keywords in vivo solid-phase microextraction；gas chromatography；pakchoi；pesticide residues；

environmental behavior；food safety

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