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Mechanisms of citrus pectin oligosaccharides regulating cholesterol
metabolism based on efflux，uptake and synthesis of cholesterol

HU Haijuan,PAN Siyi
College of Food Science and Technology/Key Laboratory of
Environment Correlative Dietology，Ministry of Education/Hubei Key Laboratory of Fruit & Vegetable
Processing & Quality Control，Huazhong Agricultural University，Wuhan 430070，China

Abstract The mechanisms of pectin oligosaccharides H1（POS H1 ）regulating cholesterol metabolism

based on the efflux，uptake and synthesis of cholesterol were studied using the macrophage model. Results
showed that POS H1 and their microbiota fermentation metabolites（P24）promoted the efflux of cholesterol
via increasing protein expressions of key genes in the liver X receptor-α-ATP-binding cassette transporter
（ABC）A1/ ABCG1 pathway. Microbial degradation made POS H1 capable of inhibiting protein expressions
of scavenger receptor A and cluster differentiating 36 to suppress cholesterol uptake，while POS H1 could
not. Both POS H1 and P24 downregulated 3-hydroxy-3-methylglutaryl coenzyme-A reductase at protein ex⁃
pression levels to inhibit cholesterol synthesis. It is indicated that POS H1 regulates cholesterol metabolism
by promoting the efflux of cholesterol，inhibiting the uptake and synthesis of cholesterol，which is one of the
mechanisms by which POS H1 regulates cholesterol metabolism，and this mechanism is related to the fermen⁃
tation of POS H1 by gut microbiota.
Keywords citrus pectin；pectin oligosaccharides；gut microbiota；cholesterol metabolism；regulatory
mechanisms

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