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Determing contents of anthocyanin and phenolic acid

compounds in black rice variety‘Huamoxiang 5’grown
in different locations with HPLC-MS

1,2
1
2
YANG Zenan ,ZHANG Qinglu ,LI Kaikai ,CHEN Hao 2
1.Key Laboratory of Environment Correlative Dietology，Ministry of Education，
Huazhong Agricultural University，Wuhan 430070，China；
2.National Key Laboratory of Crop Genetic Improvement，Huazhong Agricultural University，
Wuhan 430070，China

Abstract ‘Huamoxiang 5’is an excellent black rice variety developed by Huazhong Agricultural
University. We planted‘Huamoxiang 5’in 14 locations including Wuhan，Xi’an，Lingshui，and Ningbo etc
to study the effects of planting locations on the contents of anthocyanins and phenolic acid compounds. The
rice flour samples of‘Huamoxiang 5’were extracted and analyzed by High Pressure Liquid Chromatogra⁃
phy - tandem Mass Spectrometry（HPLC-MS），and the data were quantitatively determined with the exter⁃
nal standard method. The results showed that the contents of anthocyanins and phenolic acid compounds in
‘Huamoxiang 5’varied significantly among samples collected from different planting locations. Among
them，the contents of anthocyanins and phenolic acid compounds in the sample planted in Xi’an was the
highest，with the content of cyanidin-3-O-glucoside，peonidin-3-O-glucoside and protocatechuic acid up to
2.855 mg/g，0.491 mg/g and 0.088 mg/g，respectively. The contents of anthocyanins and phenolic acid
compounds in the sample in Huangshi was the lowest，with the content of cyanidin-3-O-glucoside，peonidin-

3-O-glucoside and protocatechuic acid of 0.865 mg/g，0.162 mg/g，and 0.048 mg/g，respectively. It is indi⁃
cated that the contents of anthocyanins and phenolic acid compounds in black rice are significantly affected
by the planting environment. Thus，selecting suitable planting locations for specific black rice varieties is im⁃
portant to produce the black rice with high nutritional value.
Keywords black rice variety‘Huamoxiang 5’；anthocyanins；phenolic acid compounds；regional dif⁃

ference；plant environment

（责任编辑：张志钰）