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第 5 期 张梦婷 等：表没食子儿茶素没食子酸酯和茶黄素对秀丽隐杆线虫耐温性的影响 239

pling［J］.Food science & nutrition，2020，8（12）：6633-6642. Progress of study on mitochondrial uncoupler［J］.Chinese bulle⁃
［29］ 金甲，张凤，杨玲玲，等 . 线粒体解偶联剂的研究进展［J］. 生命 tin of life sciences，2013，25（7）：707-715（in Chinese with Eng⁃
科学，2013，25（7）：707-715.JIN J，ZHANG F，YANG L L，et al. lish abstract）.

Effects of epigallocatechin gallate and theaflavin on temperature
tolerance of Caenorhabditis elegans

ZHANG Mengting,LI Bin,TIAN Jing
College of Food Science and Technology，Huazhong Agricultural University/Key Laboratory of Environ⁃
mental Correlative Dietology，Ministry of Education，Wuhan 430070，China

Abstract In order to investigate the effect and underlying mechanism of representative components of
green tea and black tea on nematodes（Caenorhabditis elegans）at different temperature，the survival rate of
nematodes at 4 ℃ and 35 ℃ were quantified in the presence of 0.25，2.5 and 25 μmol/L epigallocatechin
gallate（EGCG）or theaflavin（TF1）. Then，fat content and mitochondrial membrane potentials were in⁃
vestigated in nematodes incubated with 25 μmol/L EGCG and TF at 4 ℃ ，20 ℃ and 30 ℃ were deter⁃
mined. The results showed that 25 μmol/L EGCG increased the maximum and median lifespan of heat
stressed nematodes by 8.15% and 22.44%，respectively，and decreased the maximum and median lifespan
of cold stressed nematodes by 20.25% and 17.94%，respectively. 25 μmol/L TF1 had no significant effect
on the survival rate of heat-stressed nematodes，but increased the maximum and median lifespan of cold-
stressed nematodes by 9.43% and 19.01%，respectively. At 4 ℃ ，both EGCG and TF1 incubation in⁃
creased the fat content of nematodes and TF1 incubation decreased the mitochondrial membrane potential
of nematodes. At 20 ℃，EGCG and TF1 showed significant lipid-lowering effect and could significantly im⁃
prove the mitochondrial activity in senescent nematodes after long-term incubation. At 30 ℃ ，EGCG and
TF1 increased the fat content in nematodes. The results indicated that TF1 improves the survival rate of
nematodes by promoting mitochondrial thermogenesis rather than fat mobilization under low temperature
conditions，whereas EGCG incubation can induce nematode death.
Keywords Caenorhabditis elegans；epigallocatechin gallate；theaflavin；temperature tolerance；mito⁃

chondrial membrane potential

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