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290 华 中 农 业 大 学 学 报 第 41 卷

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Development and application of synchronous and rapid test strip
array device for detecting multiple mycotoxins

2
1
1
XU Zhenghua ,YANG Hanjie ,WU Sichao ,ZHANG Yi 2
1. Huangpu Customs Technology Center of the People’s Republic of China，Guangzhou 510770，China；
2.State Key Laboratory of Food Science and Technology/
College of Food Science and Technology，Jiangnan University，Wuxi 214122，China

Abstract An array device for synchronously and rapidly detecting multiple mycotoxins was developed
to further reduce the time of detection and achieve rapid customs clearance of import and export food. Due
to the interaction between probe and analyte，it is difficult to quantify the existing scheme of fixing multiple
detection lines on a single test paper，and single quality control line cannot guarantee the effectiveness of
multiple channels，and the real“synchronous”detection is not achieved. In this article，two kinds of horizon⁃
tal and vertical arrays adapted to colloidal gold lateral flow immunoassay（LFIA）strips were designed and
constructed by 3D printing technology，with four channels synchronous detection capability. The LFIA strip
of aflatoxin B1（AFB1），zearalenone（ZEN），ochratoxin A（OTA）and deoxynienol（DON）was inte⁃
grated into the array and used to detect corn，corn meal and wheat kernel. Results showed that the detection
limit（LOD）of the four toxins was 0.031 ng/mL，0.19 ng/mL，0.78 ng/mL，0.22 ng/mL，respectively，
with recoveries ranging from 74.42% to 112.40%. There was no cross-reaction. The synchronous detection
of the four indicators of a single sample，including the pretreatment time，was completed within 40 minutes.
The array device constructed will provide a potential equipment support for synchronous and rapid detection
of multiple mycotoxins.
Keywords mycotoxin；lateral flow immunoassay（LFIA）；3D printing；rapid detection device；food
security

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