Page 288 - 《华中农业大学学报（自然科学版）》2022年第5期

282 华 中 农 业 大 学 学 报 第 41 卷

Development of a chemiluminescent optical fiber immunosensor and
its application in rapid detection of deoxynivalenol in grains

HUANG Wei,HE Huiyu,LU Peng,CHEN Yiping
College of Food Science and Technology，Huazhong Agricultural University/ Key Laboratory of Environ⁃
ment Correlative Dietology，Ministry of Education，Wuhan 430070，China

Abstract Deoxynivalenol（DON），as a trichothecenes metabolite produced by Fusarium，widely ex⁃
ists in grains including wheat，rice and corn，which seriously threatens human health.Therefore，based on
the theoretical guidance of biochemistry，immunology and materials science，we constructed a chemilumines⁃
cent fiber optic immunosensor for the on-site rapid detection of DON in grains by using a combination of bi⁃
osensing recognition and chemiluminescence technology.At the same time，a portable sensing platform for
detecting the chemiluminescence signal was integrated into a black light-proof cassette of 32 cm×15 cm×
25 cm，and the weight was less than 3 kg.The results showed that Fourier transform infrared，energy dis⁃
persive spectrometry and atomic force microscopy confirmed the successful preparation of fully antigenicized
fiber probes. In addition，the constructed chemiluminescence optical fiber immunosensor had a good linear
relationship between 0.1-1 200 ng/mL，with the detection limit of 62.7 pg/mL.The functionalized optical fi⁃
ber probe can be stably stored for 30 days and has good specificity and anti-interference.The recovery rate
of DON applied to grain samples was 81.00%-107.33%，and the relative standard deviation was less than
8.69%. Compared with ELISA，the sensitivity of this method was increased by 30 times，and the linear
range was broadened by 3 orders of magnitude，indicating that it is suitable for the on-site rapid detection of
DON in grain samples.
Keywords deoxynivalenol（DON）；optical fiber immunosensor；chemiluminescence；immunoassay；

on-site rapid detection；food security

（责任编辑：赵琳琳）