Page 237 - 《华中农业大学学报（自然科学版）》2022年第5期

第 5 期 胥婷婷 等：生姜提取物缓解秀丽隐杆线虫热胁迫的研究 231

Study on alleviating thermal stress of Caenorhabditis
elegans by ginger extract

XU Tingting,TAO Mingfang,LI Rong,XU Xiaoyun,WU Ting
College of Food Science and Technology，Huazhong Agricultural University/
Key Laboratory of Environment Correlative Dietology，Ministry of Education/Hubei Key
Laboratory of Fruit & Vegetable Processing & Quality Control，Wuhan 430070，China

Abstract To explore the effect and mechanism of ginger extract（GE）on high temperature stress，an
acute heat stress（37 ℃）model of Caenorhabditis elegans was established.The lifespan and movement sta⁃
tus of wild-type nematodes，the lifespan of mutants under acute heat stress and the mRNA expression of
genes related to heat shock response（HSR）in wild-type nematodes under different degree of heat stress af⁃
ter GE treatment were measured.The results showed that compared with the control group（CK），30，60
and 120 μg/mL GE treatment significantly prolonged the heat-stress lifespan of wild-type nematodes by
16.96%，18.73% and 14.44%，respectively，and significantly improved the movement status of worms at
1st，2nd，and 3rd hour under heat stress.GE-mediated extension of heat-stress lifespan in wild-type nema⁃
todes depended on the transcription factor hsf-1，which regulated the heat shock response.GE intervention
significantly up-regulated the mRNA expression of the HSR-related genes，hsf-1，hsp-90，hsp-16.2，and the
protein expression of HSP-16.2 in wild-type nematodes at 20 ℃.Besides，GE had a regulatory effect on the
expression levels of HSR-related genes in wild-type nematodes with different degrees of heat stress（tran⁃
sient heat stress，continuous heat stress）.Compared with normal temperature（20 ℃），transient heat stress
significantly up-regulated the expression of hsf-1，hsp-16.2，hsp-70 and hsp-90 in nematodes，while GE treat⁃
ment significantly down-regulated the expression of HSR-related genes.Compared with normal temperature
（20 ℃），continuous heat-stress down-regulated the expression of HSR-related genes in nematodes，and GE
treatment significantly up-regulated the expression of hsp-16.2 and the expression of hsf-1 and hsp-90

showed an up-regulation trend，indicating that GE treatment reversed the effect of heat stress on the mRNA
expression of HSR-related genes to a certain extent.According to the results above，ginger extract could al⁃
leviate heat stress in C. elegans by regulating HSR.
Keywords ginger extract；thermal tolerance；heat shock response；Caenorhabditis elegans；heat

stress

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