Page 82 - 《华中农业大学学报（自然科学版）》2022年第5期

76 华 中 农 业 大 学 学 报 第 41 卷

功能分析［D］. 武汉：华中农业大学，2019.SHI C Y.Mining of abstract）.
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［23］ 林琼 .柠檬酸代谢及转运相关基因对柑橘果实酸度的调控机制 C N，et al.Effects of water stress on the fruit quality of citrate and
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Hangzhou：Zhejiang University，2015（in Chinese with English tion），2014，40（3）：281-287（in Chinese with English abstract）.

Effects and mechanisms of mechanical pruning-mediated fruit
thinning on improving quality of Ponkan fruit

1
1,4
2
2
2
SHI Ying ,ZENG Yike ,CHEN Siyi ,LI Guojing ,HUANG Xianbiao ,
1
1
3
LI Shanjun ,LI Chunlong ,XIE Zongzhou ,LIU Jihong 1
1.Key Laboratory of Horticultural Plant Biology，Ministry of Education，Huazhong Agricultural
University，Wuhan 430070，China；
2.Dangyang Extension Centre of Technology for Specialty Products，Hubei Province，
Dangyang 444100，China；
3.College of Engineering，Huazhong Agricultural University，Wuhan 430070，China；
4.Shaoyang Academy of Agricultural Sciences，Hunan Province，Shaoyang 422002，China

Abstract ‘Egan No.1’Ponkan was used to study the effects of mechanical pruning-mediated fruit
thinning on improving fruit quality to find a labor-saving way for thinning fruit in a more efficient manner.At
the same time，the expression patterns of genes associated with sucrose and citric acid metabolism were ana⁃
lyzed.The results showed that the fruit thinning speed and efficiency were substantially accelerated by me⁃
chanical pruning，leading to significant labor-saving.In addition，mechanical pruning-mediated fruit thinning
significantly increased the transverse diameters，longitudinal diameters，single fruit weight and fruit firmness
of the Ponkan fruit.Meanwhile，the proportion of large-sized fruit at the stage of ripening was significantly
increased to 57%.Mechanical pruning-mediated fruit thinning accelerated the color change of fruit peel，sig⁃
nificantly increased total soluble solids to 11.57% and reduced the content of titratable acids.The content of
sucrose was significantly increased to 52.10 mg/g. The result of quantitative real-time PCR showed that
genes involved in the synthesis of sucrose and the degradation of citric acid were significantly up-regulated
in the fruits collected from the thinned trees in comparison with the unthinned trees.Mechanical pruning-me⁃
diated fruit thinning can be used as a labor-saving fruit thinning method to improve the quality of Ponkan
fruit by up-regulating genes associated with the synthesis of sucrose and the degradation of citric acid.
Keywords Ponkan；mechanical pruning；fruit thinning；fruit quality；the metabolism of sucrose and

citric acid

（责任编辑：张志钰）